The dual centrifuge ZentriMix 380 R revolutionizes liposome preparation by enabling safe, gentle, and user-friendly in-vial homogenization. This innovative method allows for the production of small, uniform, and sterile liposomes in closed vials, significantly simplifying the process while achieving exceptionally high entrapping efficiencies (EE) for water-soluble compounds.

Thanks to its flexibility, the ZentriMix 380 R can handle both small batches and up to 40 formulations simultaneously, making it an ideal screening tool for identifying optimal lipid compositions and manufacturing parameters in a single working day.

A recent study published in Pharmaceutics confirmed the ZentriMix's suitability for lipid formulation screening. Researchers combined dual centrifugation with a streamlined EE-determination method using time-resolved fluorescence spectroscopy, eliminating the need for labor-intensive separation steps. Remarkably, the same sample can be used to assess entrapping efficiency, liposome size, and polydispersity index (PDI)—dramatically accelerating the formulation development process.

Further insights from differential scanning calorimetry (DSC) showed that a molecularly dispersed lipid film is no longer required. Lipids can be used as a macroscopic mixture, reducing both preparation time and organic solvent use. This advancement enhances sustainability while maintaining scientific precision.

The entire liposome screening workflow—including triplicate testing of 13 formulations or single-run testing of 40 formulations—can now be completed within a regular working day. The results are clearly summarized in a comprehensive liposome screening diagram, supporting faster and more informed decision-making in formulation development.